Publication for PRC1 and RACGAP1

Species	Symbol	Function*	Entrez Gene ID*	Other ID	Gene coexpression	CoexViewer
hsa	PRC1	protein regulator of cytokinesis 1	9055			[link]
hsa	RACGAP1	Rac GTPase activating protein 1	29127

Pubmed ID	Priority	Text
19468300	0.98	Prc1, phosphorylates Cyk-4.
	0.98	HsCyk-4 in lysates from purvalanol A-treated cells, a reaction requiring Plk1 activity (Figure 1C), we were able to ask whether Prc1 was required for efficient Ect2-BRCT precipitation of HsCyk-4.
	0.97	Prc1-depleted cells to generate phosphorylated HsCyk-4 provided a molecular explanation for the failure of HsCyk-4 to associate with Ect2-BRCT (Figure 6B).
	0.97	Prc1, Plk1, and centralspindlin all concentrate on a microtubule-based scaffold, Prc1-mediated bundling of microtubules may facilitate phosphorylation of HsCyk-4 by Plk1.
	0.96	Prc1 facilitates Plk1 phosphorylation of HsCyk-4 to allow recruitment of Ect2 to the central spindle where it can stimulate the local activation of RhoA. These data suggest that other mechanisms can compensate for the absence of Prc1 during bipolar cytokinesis.
	0.96	Prc1 and a microtubule scaffold are critical for robust generation of phosphorylated HsCyk-4.
	0.95	Prc1 is critical for generating significant amounts of phosphorylated HsCyk-4.
	0.93	Prc1 depletion in normally dividing cells results in a dramatically disorganized central spindle and a delocalization of cytokinetic factors such as Plk1 and HsCyk-4, rendering an examination into the localization of the phosphorylated form of HsCyk-4 equivocal.
	0.91	Prc1 colocalizes with centralspindlin and direct association with HsCyk-4 has been reported, raising the possibility that it might serve as an intermediary in the phosphorylation of HsCyk-4.
	0.91	HsCyk4, as well as Plk1 inhibition and prevention of HsCyk-4 phosphorylation, all result in complete abrogation of cortical contractility, depletion of Prc1 does not cause such a severe phenotype.
	0.90	Prc1 and a microtubule scaffold as critical regulators of RhoA activation by modulating Plk1 phosphorylation of HsCyk-4, cells depleted for Prc1 can form ingressing cleavage furrows and those depleted of microtubules retain contractility, indicating that Prc1 and microtubules are not strictly essential for RhoA activation upon mitotic exit.
	0.86	HsCyk-4 phosphorylation persisted in cells expressing a Prc1 derivative lacking the previously defined Plk1 docking sites.
	0.85	Prc1, Mklp2, centralspindlin, or an unknown factor, recruits Plk1 to the central spindle through association with its PBD, freeing the kinase domain to phosphorylate substrates at the central spindle (e.g., HsCyk-4, Ect2).
	0.80	Prc1 may create a scaffold of appropriately bundled microtubules on which Plk1 can target HsCyk-4 for phosphorylation.
	0.76	Prc1, facilitate Plk1 phosphorylation of HsCyk-4.
	0.74	HsCyk-4 and Prc1, as indicated, were synchronized in prometaphase with nocodazole, at which point cells were treated with 22.5 microM purvalanol A. Lysates and Ect2-BRCT-bound fractions were separated on SDS-PAGE and probed with antibodies to HsCyk-4, Prc1, and alpha-tubulin.
	0.68	HsCyk-4 precipitated in Prc1-depleted cells (knockdown = 21.5%+-5.0% of endogenous Prc1 levels) was significantly decreased relative to control cells (Figure 6A).
26764096	0.98	PRC1, the centralspindlin complex (using RacGAP1 as a marker), ECT2, and the CPC (using Aurora B as a marker).
	0.98	PRC1 and RacGAP1 were also localized (Supplemental Figure S2, A and B).
	0.97	PRC1 and augmin codepletion substantially diminished peripheral MTs, as well as RacGAP1 and ECT2 localization to these MTs (Figure 2, A, D, F, and I).
	0.96	RacGAP1, PRC1, ECT2, and MTs were significantly increased in the peripheral area after depletion of Aug6 (p value from the t test).
	0.95	RacGAP1, ECT2, and PRC1 was significantly increased in the proximity of the equatorial cell cortex (Figure 2, A-J).
	0.92	RacGAP1, PRC1, and Aurora B were substantially less abundant within the central area of the equatorial plane.
	0.90	PRC1, RacGAP1, MKLP1, and Aurora B, at the intercellular bridge (Figure 7B).
31822116	0.98	Rac GTPase-activating protein 1 (RACGAP1), epithelial cell transforming 2 (ECT2), NIMA-related kinase 2 (NEK2), BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), PDZ-binding kinase (PBK), DNA topoisomerase II alpha (TOP2A), abnormal spindle microtubule assembly (ASPM), hyaluronan-mediated motility receptor (HMMR), ribonucleotide reductase regulatory subunit M2 (RRM2), cyclin-dependent kinase inhibitor 3 (CDKN3), protein regulator of cytokinesis 1 (PRC1), and anillin actin-binding protein (ANLN) (Table 4).
	0.97	RACGAP1, ECT2, NEK2, BUB1B, PBK, TOP2A, ASPM, HMMR, RRM2, CDKN3, PRC1, and ANLN were upregulated in HCC, and the survival rate was lower for HCC with increased expression of these hub genes.
	0.96	PRC1, RACGAP1, and TOP2A, all of which encode proteins with high degrees in the PPI network.
	0.95	RACGAP1, ECT2, NEK2, PBK, TOP2A, ASPM, HMMR, CDKN3, PRC1, and ANLN.
28901457	0.98	RACGAP1, BIRC5 and PRC1, indicating a key role of TOP2A in HCC.
	0.97	RACGAP1, PRC1 and CEP55 alteration showed worse disease-free survival (Fig. 3B).
30158955	0.98	PRC1, (J) RACGAP1, (K) SPAG5, (L) TPX2.
	0.98	PRC1, (J) RACGAP1, (K) SPAG5, (L) TPX2.
31572440	0.98	RACGAP1, AURKA, KIAA0101, MAD2L1, ZEH2, CCNB1, BIRC5, ZWINT, NDC80, NCAPG, TOP2A, PRC1, CENPF, CENPN, FANCI, CDKN3, MND1, RNASEH2A, TYMS, CDK1, BUB1B, CCNA2, TPX2 and ANLN.
	0.96	PRC1, TPX2, CDK1, RACGAP1, TYMS, ANLN, PRIM1, NUSAP1, CENPF, SPAG5, SMC4, EZH2, FANCI.
15642749	0.98	MgcRacGAP binds PRC1, a mitotic CDK substrate, and that this binding inhibits MgcRacGAP activity toward Cdc42 in metaphase.
22825247	0.98	MgcRacGAP by Aurora B might disrupt the PRC1/MgcRacGAP complex and thus de-repress MgcRacGAP activity on Cdc42.
26088160	0.98	CYK-4 has been reported, and a physical interaction between Ase1, a yeast PRC1 homologue, and Klp9, a kinesin that is distantly related to ZEN-4/MKLP1 (although more closely to MKLP2), has also been shown.
29805517	0.97	PRC1, RACGAP1, TPX2, CDC20, and MCM4.
	0.95	PRC1, RACGAP1, TPX2, CDC20, and MCM4.
23792638	0.97	RACGAP1 (CYK4, MgcRacGAP), KIF20A (MKLP2), PLK1, Aurora B, PRC1, KIF4, ANLN (anillin), among others.
29563611	0.96	MgcRacGAP, the Rho GTPase activator ECT2, and the microtubule-bundling protein PRC1.
	0.96	MgcRacGAP and PRC1, two key factors in central spindle assembly, are reproducibly present in the GST-HIPK2(FL) pull-downs (Fig. 5c, middle lanes) but not in the control GST.
	0.96	MgcRacGAP and PRC1, suggesting a relationship between midbody localization and interaction with these central spindle factors.
	0.96	MgcRacGAP and PRC1.
	0.94	MgcRacGAP and PRC1.
	0.94	MgcRacGAP and PRC1, but lacks the kinase domain required to phosphorylate H2B at Ser14 and promote abscission.
	0.92	MgcRacGAP and PRC1 at midbody.
	0.92	MgcRacGAP, PRC1, and Aurora-B (Fig. 5f), indicating that HIPK2 recruitment temporally follows that of central spindle factors.
	0.81	MgcRacGAP and PRC1.
	0.67	MgcRacGAP and PRC1 and directly phosphorylates H2B at Ser32.
	0.66	MgcRacGAP and/or PRC1.
31068579	0.96	racgap1, prc1, and hmgb1) related to cell cycle, we checked their expression patterns, and found their expression kinetics were similar to CD168/HMMR+ MSCs, enriched on the top right of the plot (Fig. 4b).
	0.95	PRC1, and RACGAP1, are analyzed by real-time PCR.
	0.77	racgap1, prc1, and hmgb1.
	0.76	prc1, and racgap1.
22771033	0.96	PRC1, Endobrevin, MgcRacGAP and Plk1 were assessed in melanoma cells lacking Filamin A (M2/FLNA-/-) or stably reconstituted with Filamin A (A7/FLNA+/+).
	0.90	PRC1 appears to be restricted to efficient recruitment and/or retention of PRC1 at the midbody after successful recruitment of MgcRacGAP and MKLP1 to the centralspindle.
27144335	0.96	PRC1 and RACGAP1 (Supplementary Figure S3).
	0.76	PRC1 regulates formation of the midzone by stimulating PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle.
27835888	0.96	PRC1, MKLP1 RacGAP1, and Aurora-B (Supplementary Figure S5A), which are categorized into three groups according to the part of the midbody at which they localize: the bulge, the dark zone, or the flanking zone.
	0.91	PRC1 in the midbody (Figure 6A) but not with the bulge proteins MKLP1 and RacGAP1 or the flanking protein aurora-B (Supplementary Figure S5B).
31402959	0.96	RACGAP1, Rac GTPase-activating protein 1; PRC1, protein regulator of cytokinesis 1; CDKN3, cyclin-dependent kinase inhibitor 3.
	0.88	RACGAP1; (I) PRC1; and (J) CDKN3.
31586073	0.96	RacGAP1 subunits, and the PRC1-KIF4A complex.
	0.96	RacGAP1 partner and almost completely abolished its interaction with PRC1 and PP1beta, but only mildly affected the association with CIT-K (Fig. 7c).
22323288	0.96	MgcRacGAP recruitment was similarly impaired in PRC1-depleted cells (Figure 6B, j-l) relative to controls, in which MgcRacGAP was observed at the tips of polarized microtubules (Figure 6B, g-i).
30414309	0.96	PRC1 is necessary though for cytokinesis (Hu et al., 2012; Neef et al., 2007), where it phosphorylates MgcRacGAP/Cyk4 on several residues and elicits the binding of epithelial cell transforming sequence 2 (Ect2) (Burkard, Maciejowski, Rodriguez-Bravo, Repka, & Lowery, 2009; Burkard et al., 2007; Wolfe, Takaki, Petronczki, & Glotzer, 2009), a guanine nucleotide exchange factor for the small GTPase RhoA (Somers & Saint, 2003).
31160556	0.96	Rac GTPase Activating Protein 1 (RACGAP1), Epithelial Cell Transforming 2 (ECT2), Protein Regulator Of Cytokinesis 1 (PRC1) et al., which are all protein-coding genes.
19531213	0.95	MgcRacGAP, MKLP-1 and PRC1, constitute the core machinery controlling formation and maintenance of the central spindle and the actomyosin contractile ring during cytokinesis.
	0.92	MgcRacGAP and PRC1 are all known substrates for APC/Cyclosome.
20064404	0.95	MgcRacGAP with PRC1.
	0.95	PRC1 with MgcRacGAP may help facilitate the phosphorylation of MgcRacGAP by Polo-like kinase 1 (Plk1), which is necessary for the recruitment of the GEF Ect2 to the spindle midzone where it can activate Rho.
21386884	0.95	PRC1, RACGAP1) turned out to contribute to the difference in score significantly more than other genes (P-values between 0.0025 and 0.012, two-sided Mann-Whitney U test).
	0.65	PRC1, RACGAP1, ASPM, and PLK1 are upregulated in a variety of human cancers and that their overexpression often correlates with poor outcome (see for example and references therein).
12082078	0.95	PRC1 associates with and, specifically, if there is interaction and cooperation between PRC1, Cyk-4, and MKLP1 in maintaining the spindle midzone, as well as in the terminal stage of cleavage.
21565503	0.94	PRC1, MKLP1, RacGAP1, and Ect2 blocked the initial polarization.
	0.76	PRC1, MKLP1, RacGAP1, or Ect2 blocked the early polarization step; neither midzones nor furrows formed.
29433555	0.93	RACGAP1, PRC1, and PLK1 in luciferase and SF3B1 knockdown polychromatic and orthochromatic erythroblasts.
	0.93	RACGAP1, PRC1, and PLK1 as assessed by real-time PCR using beta-actin as internal calibrator.
	0.93	PRC1, PLK1, and RACGAP1 are indeed the top downregulated genes in both polychromatic and orthochromatic erythroblasts.
22621898	0.92	PRC1 and centralspindlin (MKLP1 complexed with RacGAP1), CENPE (a kinesin that normally relocalizes from kinetochores to midzones after cytokinesis onset), and CEP55 and ARF6, which are involved in furrow membrane trafficking during late cytokinesis.
	0.92	PRC1 on Thr-602 and left other Plk1 phosphorylations intact, for example, RacGAP1 on Thr-170 (Supplemental Figure S2A, yellow arrow).
	0.89	PRC1, centralspindlin (MKLP1/RacGAP1), and CENPE, which all responded to BI-2536 treatment by relocating to microtubule bundles in metaphase as they do in cytokinesis (Figure 1D).
	0.78	PRC1, MKLP1, RacGAP1, CEP55, and ARF6 relocalized on microtubule bundles as in cytokinesis; Aurora B, MKLP2, KIF4, Plk1, Ect2, RhoA, and Citron stayed at their metaphase localizations.
30674580	0.92	CYK4 (subunit of centralspindlin complex), PRC1 (MT cross-linking protein that is associated with midbody MTs), KIF4 (chromokinesin and PRC1-binding partner), and Aurora B kinase (subunit of chromosome passenger complex; Fig. 9 E and quantified in Fig. 9 F).
30702595	0.91	PRC1, RRM2, UBE2C, ZWINT, CDKN3, AURKA, and RACGAP1.
26595675	0.89	Protein regulator of cytokinesis 1/PRC1, SGOL1/SGO, MISP, BORA, BUB1B/BUBR1/MAD3L, CEP55, FBXO5/EMI1, CENPU/PBIP1, NEDD1, RACGAP1/CYK4, topoisomerase I-binding protein/Topors, p73/TP73, TP53BP1, and FOXM1.
30906836	0.86	RACGAP1, CCNB2, MLF1IP, AURKA, TOP2A, CENPA, CDK1, NCAPG, NDC80, NEK2, KIF11, KIF4A, BUB1, PRC1, CDCA8, CCNA2, CDC20, BIRC5, BUB1B, MAD2L1, ZWILCH, CKAP5, KIF18A, KIF2C, CENPE, CENPF, CENPK, KIF20A, CCNB1 are the genes found in the top cluster with high interaction.
23940115	0.85	PRC1, the centralspindlin complex comprised of the MKlp1/KIF23 kinesin-like motor protein and the Cyk-4/MgcRacGAP Rho-family GTPase regulator is important for central spindle integrity.
19468302	0.84	PRC1-depleted cells, presumably via redundant interactions with other MT-binding proteins such as MKLP2 and HsCYK-4 (Figure 2).
	0.77	PRC1, MKLP2, and HsCYK-4 (this study).
30774662	0.84	RACGAP1, FEN1, PRC1, and UBE2C were selected as hub genes, which were highly expressed in ATC tissues.
	0.66	RACGAP1, FEN1, PRC1, and UBE2C) related with ATC.
20109573	0.82	PRC-1 has also been implicated in the inhibition of MgcRacGAP - the GAP for Rho GTPase.
32256749	0.76	PRC1), KIF20A, Rac GTPase-activating protein 1 (RACGAP1), PTTG1, ubiquitin-conjugating enzyme (UBE2C), maternal embryonic leucine zipper kinase (MELK), NUSAP1, cytoskeleton-associated protein 2 like (CKAP2L) and TK1.
28059125	0.75	MgcRacGAP, while PRC1, KIF4A and CPC have segregated to both sides of NAIP (late anaphase).
	0.51	PRC1, the chromosomal passenger components INCENP, Survivin and Borealin, the Centralspindlin MgcRacGAP or BRUCE.
20148280	0.65	PRC1, and HsCYK-4, which happens when Plk1 is already activated, Plk1-dependent PBIP1 priming and binding occurs in early interphase sufficiently ahead of Plk1 activation in late G2.