Publication for SFPQ and PSPC1

Species	Symbol	Function*	Entrez Gene ID*	Other ID	Gene coexpression	CoexViewer
hsa	SFPQ	splicing factor proline and glutamine rich	6421			[link]
hsa	PSPC1	paraspeckle component 1	55269

Pubmed ID	Priority	Text
25013176	0.98	PSF associated with both ssASOs and ASO/RNA duplex, whereas PSPC1 and hnRNPK were preferentially enriched by selection with ssASOs.
	0.92	PSF and PSPC1, co-localized precisely with PS-ASOs in these filament structures (Figure 5A, Supplementary Figure S6B, and S6C).
	0.60	PSF, PSPC1 and hnRNPK, were also isolated with PS-ASOs (Figure 1C).
	0.60	PSF/PSPC1, leading to the subsequent assembly of other paraspeckle components.
	0.56	PSF, PSPC1 and hnRNPK.
31220736	0.98	PSPC1, and SFPQ with the FUS-DDIT3 oncoprotein.
	0.97	PSPC1 and SFPQ.
	0.96	PSPC1 and SFPQ.
	0.96	PSPC1) was detected further down in the interactome ranking, it was chosen for validation as a member of the drosophila behavior/human splicing (DBHS) protein family that often functions in complex with NONO and SFPQ.
	0.96	PSPC1, RBM14, SFPQ, SPAG5) that were identified in both the nuclear and whole cell lysate interactomes (Figure S8B) also ranked high in the nuclear interactome with a PES z-score > 0.5 (Figure 4B).
25694451	0.98	PSPC1/PSF levels will also be important to consider along with measuring the stoichiometry of these proteins as spermatogenesis proceeds.
	0.95	PSF nuclear speckle-positive cells followed a similar trend to PSPC1 nuclear speckles, with IMPalpha2-FL causing a significant increase and IMPalpha2DeltaIBB a significant decrease.
	0.59	PSF-positive nuclear speckles were analyzed based on preliminary data collected in HeLa cells using GFP-tagged IMPalpha2 constructs as described above for PSPC1 nuclear speckles.
30018955	0.98	PSPC1 and SFPQ shared by lncRNAs MALAT1 and NEAT1.
	0.98	SFPQ, and PSPC1 are components of paraspeckle which was the key element of HDP-RNP.
	0.98	PSPC1, paraspeckle component 1; SFPQ, splicing factor proline and glutamine rich; NONO, non-POU domain-containing octamer-binding protein; HDP-RNP, HEXIM1-DNA-PK-paraspeckle components-ribonucleoprotein complex.
24173718	0.98	PSPC1, NONO (p54nrb), and splicing factor proline/glutamine-rich (SFPQ; PSF).
	0.92	SFPQ is the only one with a characterized DNA-binding domain, found within a unique N-terminus that is distinct from NONO and PSPC1.
26571461	0.98	PSPC1 and SFPQ proteins in patients' cells compared to controls (C1 and C2).
	0.97	PSPC1 (2.22 and 2.78 fold respectively) and SFPQ (1.54- and 1.71-fold, respectively), were detected in both patients compared to controls.
27033831	0.98	PSF/SFPQ, P45NRB/NONO, and PSPC1.
28255245	0.98	PTB-associated splicing factor (PSF), and PSPC1 to the central scaffolding RNA, NEAT1.
31683819	0.98	PSPC1, NONO or SFPQ fails to support paraspeckle assembly.
32233169	0.98	PSPC1, PSF, hnRNPK, and p54nrb).
29662142	0.97	PSP1, alpha-PSF or alpha-p54nrb antibodies, we observed the presence of a band of the expected length for the HDV amplicon in the cells treated with tetracycline (Fig. 1).
	0.97	PSP1 associates, directly or indirectly through protein:protein or protein:RNA:protein interactions, with the HDV RNA genome in this cellular system, and corroborate our previous observations of the association of both PSF and p54nrb with HDV RNA.
	0.97	PSF, p54nrb and PSP1 leads to an inhibition of HDV replication
	0.97	PSF (a), PSP1 (b) and p54nrb (c) in each treatment compared to beta-actin.
	0.96	PSF, p54nrb and PSP1 in HEK-293 cells.
	0.96	PSF, PSP1 and p54nrb.
	0.95	PSF, p54nrb and PSP1 leads to a decrease of the HDV genome accumulation, indicating that these proteins are required directly or indirectly for the accumulation of HDV genomes in these cells.
	0.93	PSP1 antibody, or with either alpha-PSF or alpha-p54nrb antibodies as positive controls.
	0.91	PSF, p54nrb and PSP1 reduce HDV RNA genome accumulation.
	0.90	PSF, p54nrb and PSP1 in HDV replication, we investigated whether knockdown of these proteins would affect HDV accumulation following induction of HDAg-S production.
	0.86	PSF, p54nrb or PSP1 knockdown, we observed a decrease of more than 90% of HDV RNAs accumulation compared to the cells only treated with tetracycline (Fig. 2d).
	0.85	PSF, p54nrb and PSP1 in cells replicating HDV RNA to determine the requirement for these proteins in HDV replication.
	0.84	PSF, p54nrb or PSP1 compared to cells transfected with the pool of scramble siRNAs in cells replicating HDV (Fig. 2e).
	0.78	PSF, p54nrb and PSP1, associate with the HDV RNA genome and are required for HDV replication in HEK-293 cells.
	0.64	PSF, alpha-p54nrb and alpha-PSP1 antibodies.
	0.55	PSF, p54nrb and PSP1), and that these proteins are required for the accumulation of HDV RNA, we hypothesized that paraspeckles might be disrupted upon HDV replication.
	0.53	PSP1, alpha-PSF or alpha-p54nrb antibodies showed bands corresponding to the expected size for the NEAT1_2 amplicon (Fig. 5d).
21144806	0.97	PSF and PSPC1 are capable of interaction in vivo, we performed co-immunoprecipitation experiments using protein extracts of HeLa cells that were metabolically labeled using 35S-methionine.
	0.94	PSF in directing relocalization of PSPC1, we performed experiments in which PSF or p54nrb was overexpressed by co-transfection with the fluorescently tagged PSPC1.
	0.94	PSF, lower panel, anti-PSPC1.
	0.94	PSPC1 in the presence of excess PSF.
	0.91	PSF/SFPQ), 54 kDa RNA binding protein (p54nrb/NONO), and Paraspeckle Component 1 (PSPC1) make up the Drosophila behavior human splicing (DBHS) family.
	0.89	PSF with PSPC1 and relocalization of PSF PSPC1 complex to sites of laser-induced DNA damage.
	0.88	PSF sequences in relocalization, we investigated the ability of PSF to direct relocalization of the third family member, PSPC1.
	0.88	PSF antibody precipitated a polypeptide corresponding to p54nrb and an additional minor band migrating at a position consistent with the predicted 59 kDa size predicted for PSPC1 (Fig. 4A, lane 3).
	0.88	PSF was capable of directing the relocalization of PSPC1 to sites of DNA damage, we transfected HeLa cells with fluorescently tagged PSF and PSPC1.
	0.81	PSF-AcGFP and PSPC1-dsRed, treated, and analyzed as in (C).
	0.68	PSPC1 under each set of conditions (p54nrb depletion or PSF overexpression) and found that it was similar to PSF itself, with peak accumulation at about 120 s and a steep decline by 300 s (Fig. 4G).
	0.61	PSF, but not p54nrb, promoted relocalization of PSPC1 to sites of laser-induced DNA damage (Fig. 4E, 4F).
25855809	0.97	PSPC1 interacts with both P54nrb and PSF and was found to associate and co-localizes with PS-ASOs in nuclear paraspeckles.
	0.97	PSF or PSPC1, binds preferentially to 2'-F-modified oligonucleotides, it is possible that interaction between P54nrb and 2'-F-modified oligonucleotides may induce the degradation of P54nrb, leading to reduced stability of PSF and/or PSPC1 which associate with P54nrb.
	0.96	PSF and PSPC1 proteins by 2- to 3-fold (Figure 5E).
	0.96	PSF was observed upon simultaneous knockdown of both P54nrb and PSPC1.
	0.95	PSF protein was not reduced with double knockdown of its heterodimer partners, P54nrb and PSPC1 (Figure 5E).
	0.91	PSF and PSPC1, proteins that are functionally redundant in DSB repair.
	0.57	PSPC1 by siRNA treatment has no effect on the levels of P54nrb or PSF.
	0.51	PSF and PSPC1.
29084942	0.97	Sfpq, Pspc1, and NonO in the nucleoplasm in both human and mouse cell lines by Western and Northern blot analyses (Fig. 3a and Supplementary Fig. 4a).
	0.97	Sfpq, Pspc1, and NonO interact with mature let-7a in RA-treated P19 cells.
	0.94	Sfpq, Pspc1, and NonO co-immunoprecipitated with mature let-7a in RA-stimulated P19 cells, a cellular model for embryonic stem cells.
	0.93	Sfpq and Pspc1 interact with mature let-7a but not with the precursor.
	0.93	Sfpq, Pspc1, or NonO. RAW 264.7 cells were transfected with the indicated siRNAs.
	0.89	Sfpq knockdown also inhibited the interaction between let-7a and either Pspc1 or NonO, whereas Pspc1 or NonO knockdown did not affect the interaction between Sfpq and let-7a (Fig. 2b and Supplementary Fig. 3c).
	0.78	Sfpq-Pspc1-NonO complex could regulate miRNA functions in an RNA-dependent fashion.
	0.61	Sfpq mediates the interaction between miRISC and Pspc1 or NonO. a Co-IP of endogenous Ago2 with Sfpq, Pspc1, or NonO from P19 cells.
28846091	0.97	PSF (aka SFPQ), PSPC1 (aka PSP1), all of which are key RBP constituents of paraspeckles, and the other class consists of ILF3 (aka NF90) and ILF2 (aka NF45) previously implicated in nuclear export of a viral dsRNA.
	0.97	PSF, but not PSPC1, reduced the expression of all miRNAs from the miR-17-92a locus with corresponding increase in their pri-miRNA in HeLa cells (Fig. 1b, c; Supplementary Fig. 2a-c).
	0.97	PSF and the Microprocessor, we performed siRNA knockdown (Fig. 4d), finding that NEAT1 knockdown largely abolished the in vivo interactions of NONO with the Microprocessor without affecting its interaction with PSPC1 (Fig. 4e), and by contrast, MALAT1 knockdown showed no impact on any of these interactions (Fig. 4f).
	0.92	PSF, and NEAT1 (both V1 and V2), and again, PSPC1 knockdown showed the opposite effect on many miRNAs (Fig. 1e, f).
	0.83	PSF, but not PSPC1, are required for the structural integrity of paraspeckles, which we also confirmed (Supplementary Fig. 3a), we chose to first focus on understanding the mechanism for these two paraspeckle components to stimulate pri-miRNA processing.
	0.61	PSF and NEAT1, but not PSPC1, in pri-miRNA processing.
24819514	0.97	PSPC1 expression can be induced by cisplatin as well as evidence that the other two paraspeckle proteins, PSF and p54nrb, are involved in DNA repair, all lead to the hypothesis that PSPC1 is very likely a participant in the DDR.
	0.96	PSF could promote the recruitment of PSPC1 to sites of DNA damage following knockdown of p54nrb.
	0.90	PSF and p54nrb, PSPC1 forms the protein core of paraspeckles.
	0.89	PSF and p54nrb play important roles in DNA repair during DDR, we were wondering whether PSPC1 also had a similar function.
	0.53	PSPC1 can dimerize with p54nrb through the coiled-coil domain to regulate pre-RNA processing, but not with PSF.
30445466	0.97	SFPQ) and paraspeckle protein component 1 (PSPC1).
	0.93	SFPQ and PSPC1, we transfected HeLa cells with plasmid encoding FLAG-NONO, FLAG-SFPQ or FLAG-PSCP1 and irradiated with UV (30 J/m2).
	0.82	SFPQ, FLAG-NONO or FLAG-PSPC1.
	0.82	SFPQ or PSPC1 by co-immunoprecipitation analysis.
	0.61	SFPQ or PSPC1.
24334610	0.97	PSP1 and PSF are recruited into FUS aggregates in neuroblastoma SH-SY5Y (C and E, arrows) and COS7 (D, arrows) cells.
	0.97	PSP1 and another core paraspeckle protein, PSF (polypyrimidine tract-binding protein-associated splicing factor), also accumulated within the cytosolic FUS aggregates in SH-SY5Y and COS7 cells (Fig. 6C-E, arrows).
	0.95	paraspeckle protein 1 (PSP1) and PSF.
	0.83	PSF and PSP1.
29535823	0.97	SFPQ, PSPC1, and p54nrb/NONO have been shown to interact with transcription factors, DNA, and RNA, to exert various functions.
	0.95	PTB-associated splicing factor (PSF) and paraspeckle protein component 1 (PSPC1); these proteins perform specific functions by forming homo- or heterodimers.
	0.91	PSPC1 paralog and formation of a functional heterodimer with SFPQ during DNA repair.
29358041	0.97	SFPQ and PSPC1.
	0.96	SFPQ, and PSPC1, together with other pre-mRNA splicing factors, transcription factors, and heterogeneous nuclear ribonucleoproteins (hnRNPs), are also components of paraspeckles - subnuclear bodies assembled on the long non-coding RNA Neat1.
30153828	0.97	SFPQ (splicing factor proline/glutamine-rich), NONO (p54nrb), and PSPC1 (paraspeckle component 1) are protein components of paraspeckles.
	0.90	SFPQ, but not p54nrb or PSPC1, prevented the apoptotic effects induced by IM in K562 cells (Fig. 3b).
20881053	0.97	PSPC1, P54NRB/NONO, and SFPQ/PSF.
25712094	0.97	PSF and PSPC1 increased ASO activity.
27571227	0.97	PSF (SFPQ), and PSPC1 can bind to PS ASOs, resulting in inhibition of ASO directed RNAseH1-mediated activity.
28673861	0.96	SFPQ, P54nrb, PSPC1 and GAPDH.
	0.94	SFPQ, P54nrb and PSPC1) in two different large datasets including Curtis (1700 cases) and TCGA (450 case) (Fig. 3C-H).
	0.94	SFPQ, PSPC1 and P54nrb) by co-immunoprecipitation experiments.
	0.93	SFPQ, P54nrb and PSPC1 mRNA levels in association with patient outcome using RFS as an end point in breast cancer patients (3554, 1660 and 3554 respectively) (KM-plotter database).
	0.91	PSPC1), splicing factor proline and glutamine-rich (SFPQ) and non-POU domain containing octamer binding (P54nrb).
	0.89	SFPQ, P54nrb and PSPC1 mRNA expression levels in 61 normal and 389 invasive breast cancer cases using TCGA dataset of ONCOMINE database.
	0.86	SFPQ, P54nrb and PSPC1 and mRNA levels in 144 normal and 1556 invasive breast cancer cases using Curtis dataset of ONCOMINE database.
	0.61	SFPQ, P54nrb and PSPC1 as well as NEAT1 RNA in the less aggressive luminal A MCF7 cells in comparison to the more aggressive MDA-MB-231 cells.
	0.60	SFPQ, P54nrb and PSPC1 mRNA levels in association with patient outcome using DMFS as an end point in breast cancer patients (1609, 664 and 1609 respectively) (KM-plotter database).
30717168	0.96	PSF/SFPQ, PSPC1, and p54nrb/NONO.
	0.96	PSPC1; yellow), and polypyrimidine tract-binding protein PTB-associated splicing factor/splicing factor proline glutamine rich (PSF/SFPQ; brown).
	0.96	PSF/SFPQ, and PSPC1 (as well as other paraspeckle proteins; not shown in figure) are distributed within the structure in consideration of the before established binding domains on NEAT1.
	0.94	PSF/SFPQ, PSPC1, and p54nrb/NONO, indicating a crucial role of paraspeckles in HDV infection.
26727894	0.96	PSPC1 and SFPQ.
	0.94	PSPC1, PSF/SFPQ, and the long non-coding RNAs (lcnRNA) NEAT1/Men epsilon/beta and Ctn are integral components of paraspeckles.
	0.93	PSPC1 and NONO or SFPQ and NONO.
28288210	0.96	PSF has additional functions not ascribed to P54nrb and PSPC1, which explains the upregulated p21Waf1/Cip1 and Gadd45a mRNAs in HeLa cells depleted of PSF but not P54nrb or PSPC1.
	0.79	PSF, PSPC1, hnRNPK, FUS and TDP43, in an ASO dose-dependent manner.
29033980	0.96	SFPQ/PSF, which leads to the secondary recruitment of the short lncRNA isoform NEAT1_1 and PSPC1, and the further loading of other paraspeckle protein components.
	0.93	PSPC1), non-POU domain containing, octamer binding (p54nrb/NONO), and splicing factor proline/glutamine-rich (SFPQ/PSF).
23362321	0.96	PSF-expressing plasmid for 24 h. The cells were then visualized by confocal microscopy for GFP-PSF (green), endogenous PSP1 stained with specific antibody (red), and nuclei stained with 4',6-diamidino-2-phenylindole (DAPI) (blue).
24233053	0.96	PSPC1, PSF (SFPQ) and p54nrb (NONO).
29390093	0.96	PSF and PSPC1 proteins were observed in intellectual disability patients carrying a nonsense mutation in P54nrb.
29462943	0.96	SFPQ/PSF, and PSPC1.
32106418	0.96	PTB-associated splicing factor (PSF)/splicing factor proline/glutamine rich (SFPQ), Non-POU domain-containing octamer-binding protein (NONO)/nuclear RNA-binding protein, 54 kDa (p54nrb) and paraspeckle component 1 (PSPC1)/paraspeckle protein 1 (PSP1).
28240251	0.95	SFPQ in paraspeckles, thereby stabilizing NEAT1 RNA, and enabling higher levels of PSPC1 recruitment and accumulation into paraspeckles.
	0.95	PSPC1/SFPQ into the nucleus/paraspeckles was investigated.
	0.95	PSPC1/SFPQ or exogenous DsRed2-PSPC1) nuclear transport and paraspeckle localization in HeLa cells.
	0.94	SFPQ and PSPC1 as paraspeckle markers.
	0.94	PSPC1 or SFPQ nuclear accumulation to be determined following resonance scanning.
	0.93	PSPC1 (A), using indirect immunofluorescence with an Alexa Fluor 546 (A546) secondary antibody to detect endogenous SFPQ (B) or through exogenous PSPC1 by co-transfecting with a plasmid encoding DsRed2-PSPC1 (C).
	0.89	PSPC1 Fn/c values in the IMPalpha2 siRNA knockdown samples were reduced to ~80% of their scrambled counterparts (PSPC1: 0.78 and 0.80; SFPQ: 0.81 and 0.94; calculated from values in Supplementary Tables S8-S15).
	0.77	PSPC1 and SFPQ nuclear accumulation and their localization to paraspeckles.
	0.77	PSPC1, B:SFPQ, C:DsRed2-PSPC1) were "% cells positive for foci", "cytoplasmic PSM intensity", "nuclear PSM intensity", "PSM Fn/c per cell", "PSM intensity per cell", "number of nuclear foci per cell", "sum volume of nuclear foci per cell", "sum nuclear foci PSM intensity per cell", "nuclear foci volume", "nuclear foci PSM intensity" and "sum nuclear foci PSM intensity".
	0.71	PSPC1 nuclear accumulation and localization into paraspeckles also affected another core DBHS paraspeckle marker, we examined endogenous SFPQ in HeLa cells transiently transfected to express GFP-tagged IMPalpha constructs.
	0.62	PSPC1 [endogenous and exogenous] and SFPQ [endogenous only]).
	0.62	SFPQ localization to nuclear foci in a manner similar to that recorded for PSPC1 localization (Table 2 and Fig. 3B).
	0.62	PSPC1 or SFPQ (each in duplicate experiments) and the relevant IMPalpha by indirect immunofluorescence (Supplementary Tables S8-S15).
	0.59	PSPC1 was detected, and 0.65 and 0.87 for SFPQ samples (calculated from values in Supplementary Tables S8-S15), demonstrating effective IMPalpha2-targeting by these siRNAs.
25832716	0.95	PSF, together with Matrin 3, PSPC1, and p54nrb/NONO, binds with high affinity to hyper A-to-I edited mRNAs, presumably through specific recognition of the inosines.46 This high-affinity interaction anchors hyperedited RNAs within paraspeckles and prevents their export to the cytoplasm.8, 46 Notably, such PSF-dependent nuclear retention has been observed to be relieved either by loss of NEAT1 expression and concomitant dissociation of paraspeckles,8 or by specific cleavage of the inosine-containing portion of the message, typically in an extended 3'UTRs.48 Thus, nuclear retention by PSF is an important regulatory layer in determining the export and expression of mammalian mRNAs.
	0.94	PSF expression by as little as twofold to threefold induces rapid apoptosis.10, 11 Conversely, p54nrb/NONO is readily knocked down with little phenotype, and some mammalian cell types do not express detectable amounts of PSPC1.11, 12, 13 In zebrafish, PSF is necessary for general cell survival and for neuronal development, while in mice even modest depletion of PSF in thymocytes is sufficient to block T-cell development.10, 14 Moreover, somatic mutations in the gene encoding PSF, or gene fusion events between PSF and other proteins, have been linked to multiple diseases including autism,15 Alzheimer's disease,16 renal cell carcinoma,17 acute myeloid and lymphoblastic leukemia,18, 19 and prostate cancer.20 Whether the expression or function of PSF is altered in these disease states remains to be determined.
	0.93	PSF has also been implicated in functions not ascribed to p54nrb/NONO or PSPC1.
	0.92	PSF in paraspeckles, as this is a definitive feature of DBHS proteins.3 Paraspeckles are subnuclear bodies that are often present adjacent to, but distinct from, speckles and are defined by the presence of the NEAT1 (nuclear-enriched abundant transcript 1) noncoding RNA (ncRNA) and the DHBS proteins.3, 8 Knockdown studies have shown that both PSF and p54nrb/NONO are required for the formation of paraspeckles, while PSPC1 is less critical for paraspeckle formation and may localize to these structures as a consequence of the protein-protein interactions among all the DHBS proteins.9 The structure, regulation, and function of paraspeckles have been reviewed elsewhere,3, 7 so are not covered in depth here.
	0.76	PSF has not yet been published, a structure of the RRMs of the other human DBHS proteins was reported in 2012 from a co-crystal of p54nrb/NONO and PSPC1, which included both RRMs of each protein plus additional sequences that comprise the DBHS core4 (NOPS and coiled-coil, see below).
27084935	0.95	PSPC1 to form a functional heterodimer with SFPQ in DNA repair.
	0.94	SFPQ, NONO and PSPC1 as fundamentally dimeric means that some past literature, where they are annotated as individual functional units, may need to be reinterpreted.
	0.94	SFPQ are more abundant than PSPC1.
	0.94	SFPQ and NONO do not compensate for the loss of PSPC1 and PSPC1 and SFPQ cannot compensate for the loss of NONO in intellectual disability in humans.
	0.85	SFPQ (4WII) and PSPC1/NONO (3SDE).
23267412	0.95	PSF and PSP1 proteins.
	0.60	PSF and p54, followed by subsequent recruitment of PSP1 and NEAT1_V1.
27783096	0.95	PSPC1, SFPQ functions as a competitive inhibitor of STAT3 in STAT3-mediated HSV-1 gene expression, suggesting that SFPQ may play a vital role in the host antiviral response.
	0.81	PSPC1 (paraspeckle component 1), NONO (non-POU domain-containing octamer-binding, p54), SFPQ (splicing factor, proline- and glutamine-rich), CPSF6, and RNA-binding motif 14 (RBM14), and dozens of new paraspeckle proteins have recently been identified.
30254462	0.95	SFPQ, NONO, and PSPC1 are three characteristic paraspeckle proteins that have been proposed to facilitate DNA damage repair and the consequential chemo- or radio-resistance in cancer cells via seemingly different mechanisms.
	0.93	PSPC1, SFPQ, and NONO, all of which belong to the DBHS protein family and are fundamentally and dynamically dimerized.
30430751	0.95	PSF/SFPQ; Prasanth et al., 2005), 54 kDa nuclear RNA- and DNA-binding protein/Non-POU domain-containing octamer-binding protein (p54NRB/NONO) and paraspeckle component 1 (PSPC1; Fox et al., 2002), and the lncRNA NEAT1.
23835137	0.94	PSF ((D), ALS-Pt C), PSP1 (upper in (E), ALS-Pt D) and p54nrb (lower in (E), ALS-Pt B) overlap with NEAT1_2 foci.
	0.93	PSF and PSP1 (Figure 2D).
	0.90	paraspeckle protein-1 (PSP1)/paraspeckle component1, p54nrb/non-POU domain-containing octamer-binding protein (NONO), polypyrimidine tract binding protein-associated splicing factor (PSF), RNA polymerase II and other proteins.
	0.87	PSF, PSP1, and p54nrb were colocalized with NEAT1_2 foci in the nuclei of ALS motor neurons (Figure 4D and E, and Additional file 4: Figure S4).
	0.78	PSF, PSP1 and RNA polymerase II.
	0.73	PSF or anti-PSP1 antibodies were used.
25765647	0.94	SFPQ is conspicuously divergent from NONO and PSPC1 (Supplementary Figure S4).
	0.94	PSPC1 (PSPC1-61-340, schematically marked as protein '1' in Figure 7a) contains the dimerization domain as well as the equivalent residues to the SFPQ coiled-coil interaction motif (Figure 2a).
	0.93	SFPQ, NONO and PSPC1.
	0.89	SFPQ, different with respect to the related proteins NONO and PSPC1, is a unique region immediately N-terminal to the RRM1 that encodes a putative DBD.
26261210	0.94	PSPC1, or the nuclear matrix protein Matrin 3, co-precipitated with PSF from stimulated (S) versus unstimulated (U) cells, despite a marked difference in TRAP150 co-precipitation (Figure 5C).
28035991	0.94	SFPQ/PSF and other paraspeckle protein components, 54 kDa nuclear RNA-binding protein (P54nrb) and paraspeckle protein 1 (PSPC1).
28813667	0.94	SFPQ and PSPC1 were among the most strongly detected proteins obtained from samples that had been treated with DNase only (Fig. 1D-F).
19720872	0.93	PSPC1, P54NRB/NONO, or PSF/SFPQ.
	0.81	PSF/SFPQ, P54NRB/NONO, and PSPC1 all copurify NEAT1 RNA to varying levels.
30380741	0.93	SFPQ/PSPC1, which dissociate upon stimulation with ISD (interferon stimulatory DNA) and then are available for the DDR.
31311324	0.93	PSPC1, non-POU domain containing octamer binding (NONO), and splicing factor proline and glutamine rich (SFPQ).
21170033	0.92	PSP1 and p54nrb, n=5 for PSF, and n=12 for Men epsilon/beta ncRNAs, mean+-s.e.m.).
	0.87	PSF was immunoprecipitated by anti-PSF antibody and both PSP1 and p54nrb were detected in the IP by immunoblotting (Fig. 1c).
	0.81	PSP1 protein to a specific locus (arrow) in the C2C12 nucleus recruited p54nrb, but not Men epsilon/beta ncRNAs, PSP1, PSF, or PSP2 protein therefore failing to form paraspeckles.
	0.78	PSP1, mCherry-p54nrb was recruited to the tethering site, but mCherry-PSP1, PSP2, endogenous PSF, or Men epsilon/beta failed to be recruited (Fig. 1a).
	0.73	PSP1, p54nrb, and PSF) showed similar kinetics during de novo paraspeckle assembly upon DOX induction (Fig. 5b).
	0.52	PSP1, p54nrb, PSF, and PSP2 (Fig. 2d and Supplementary Information, Figs. S2, S3 and Movies 4, 5), but not SC35 and SF2/ASF, two major RNA binding proteins enriched in nuclear speckles and not found in endogenous paraspeckles (Fig. 2d and Supplementary Information, Fig. S4).
26441864	0.92	(PSP1, PSF, and p54nrb); or PML bodies (PML and SUMO1).
	0.82	s (p54nrb, PSF, and PSP1) or PML bodies (PML and SUMO1) (Figures 2B,C).
27646270	0.92	SFPQ, PSPC1, NONO, and FUS.
	0.87	SFPQ, NONO, PSPC1, and FUS are the core components of paraspeckles, which exclusively colocalized with the middle region of Neat1_2 in the center of paraspeckles.
32034402	0.90	PSPC1) from the Drosophila behavior human splicing (DBHS) protein family, SFPQ is found predominantly in the nucleus and has been implicated in a wide range of physiological functions including neuronal differentiation and development.
28977508	0.89	PSF, FUS, and PSPC1.
21680045	0.88	PSP1, p54nrb and PSF) show a similar mobile fraction (60-70%) within the paraspeckle.
	0.75	PSP1, p54nrb, PSF and PSP2) were efficiently recruited to the transcription sites.
25155612	0.88	PSPC1 and PSF) or nuclear speckles (SRSF1) using immunoblotting of CHART-enriched extracts (Figure 7A; see also).
	0.62	PSPC1 and PSF, the nuclear speckle component SRSF1, and a chromatin-associated protein, histone H3.
28498981	0.86	PSP1 (paraspeckle protein 1), p54/nrb (Nono), the splicing factor SFPQ (PSF) and probably also TDP-43, which associate around the long non-coding RNA (lncRNA) NEAT1, for all of which an association with immune cell function has been described.
29706968	0.86	SFPQ, PSPC1, RBM14, and NONO.
30906827	0.84	PSF/SFPQ, P54NRB/NONO, and PSPC1 where lncRNAs such as NEAT1 may act as platforms for such nuclear organization.
25414336	0.83	PSF (PTB-associated splicing factor), p54nrb, PSP1 and RBM14/ CoAA/PSP2 (hereafter referred to CoAA).
27165283	0.83	paraspeckle protein 1 (PSP1); splicing factor, proline- and glutamine-rich (SFPQ) protein; and p54nrb/NONO (non-POU-domain-containing octamer-binding protein).
27665741	0.79	SFPQ and PSP1, paraspeckles also contain an additional ~40 or more PSPs.
22941645	0.78	SFPQ and PSPC1, is strongly associated with the chromatin and nearly absent in the nuclear soluble and cytoplasmic fractions.
	0.65	PSPC1 and SFPQ).
28977560	0.59	PSF-Top1 trimers were reduced in cells that overexpress RNase H1, since protein levels of PSPC1 were not affected by RNase H1 overexpression.
30072977	0.57	SFPQ) protein, paraspeckle component 1 (PSPC1), and a non-POU domain containing, octamer-binding NONO/p54nrb protein.
27816966	0.54	PSPC1) and splicing factor, proline- and glutamine-rich (SFPQ), which are localized into the nucleus and are involved in various aspects of RNA metabolism.