Publication for Nodal and Pou5f1

Species	Symbol	Function*	Entrez Gene ID*	Other ID	Gene coexpression	CoexViewer
mmu	Nodal	nodal	18119			[link]
mmu	Pou5f1	POU domain, class 5, transcription factor 1	18999

Pubmed ID	Priority	Text
19688838	0.99	Nodal knockdown by its siRNAs resulted in a marked reduction of Oct-4 mRNA, indicating that autocrine Nodal stimulates Oct-4 transcription.
	0.98	Nodal was identified as the first autocrine signaling molecule that promotes proliferation of mouse spermatogonial stem/progenitor cells via Smad2/3 and Oct-4 activation.
	0.98	Nodal plays an essential role in regulating proliferation of mouse spermatogonial stem/progenitor cells via Smad2/3 phosphorylation and Oct-4 transcription.
	0.98	Nodal was coexpressed with Oct-4, a spermatogonial stem/progenitor cell marker , in a subpopulation of type A spermatogonia, reflecting that Nodal-positive cells within the seminiferous tubules are likely stem/progenitor cells and that a Nodal/Oct-4 signaling pathway could be important in regulating their proliferation and maintenance.
	0.98	Oct-4 transcription by Nodal stimulation was totally interrupted by pretreatment with the inhibitor SB431542, suggesting that Nodal activates Oct-4 transcription in mouse spermatogonial stem/progenitor cells.
	0.97	Nodal activates Smad2/3 phosphorylation, Oct-4 transcription, cyclin D1, and cyclin E expression, whereas SB431542 completely abolishes their increase.
	0.97	Nodal Activates Smad2/3 Phosphorylation and Oct-4 Transcription in C18-4 cells and Nodal Knockdown Results in a Reduction of Oct-4 Transcription
	0.97	Nodal activates the transcription of Oct-4 in stem cells .
	0.97	Nodal signaling activates Smad2/3 phosphorylation, Oct-4 transcription, cyclin D1 and cyclin E but not cyclin A expression in C18-4 cells.
	0.96	Nodal Signaling via an Autocrine Pathway Promotes Proliferation of Mouse Spermatogonial Stem/Progenitor Cells through Smad2/3 and Oct-4 Activation
	0.96	Nodal was coexpressed with Oct-4 (Fig. 3A) and with GFRA1 (Fig. 3B) in type A spermatogonia from 6-day-old mice.
	0.96	Oct-4 transcription in C18-4 cells treatment with Nodal for the indicated periods.
	0.95	Oct-4 was reduced in C18-4 cells treated with Nodal siRNAs compared to no siRNA groups (Fig. 5C, 5D).
	0.95	Oct-4 mRNA was increased in C18-4 cells treated with Nodal for 15 minutes (7.7 +- 0.6 fold, n = 3), 30 minutes (8.0 +- 0.7 fold, n = 3), and 1 hour (5.4 +- 0.4 fold, n = 3) (Fig. 6B, 6C).
	0.95	Nodal-induced transcription of Oct-4 at 1 hour (Fig. 6B, 6C), which is consistent with our above observations that the transcription of Oct-4 was reduced in C18-4 cells treated with Nodal siRNAs compared to no siRNA groups.
	0.92	Oct-4 mRNA by Nodal in C18-4 cells was completely abolished by pretreatment with SB431542 (Fig. 6B, 6C).
	0.90	Nodal with Oct-4 (A) and Nodal with GFRA1 (B) in a subpopulation of type A spermatogonia from 6-day-old mice.
	0.89	Nodal and Oct-4 or Nodal and GFRA1.
	0.86	Nodal with Oct-4 and GFRA1 in type A spermatogonia of 6-day-old mice and its absence in Sertoli cells, as well as expression of Nodal receptors ALK4 and ActR-IIB in type A spermatogonia of immature mice and in C18-4 cells.
21731500	0.99	Nodal/Activin signaling directly controls the Oct4 master regulator of pluripotency by graded phospho-Smad2 binding in the promoter region.
	0.99	Oct4 Is a Direct Target of Nodal/Activin Signaling via Graded Phospho-Smad2 Recruitment to the Promoter.
	0.98	Nodal/Activin signaling instructions via a corresponding gradient of Smad2 phosphorylation that selectively titrates self-renewal against alternative differentiation programs by direct regulation of distinct target gene subsets and Oct4 expression.
	0.98	Oct4, a master gene controlling the stem cell state thereby reconciling the opposing functions of the Nodal/Activin pathway in differentiation versus self-renewal programs.
	0.98	Oct4 were also repressed by inhibition of Nodal/Activin signaling.
	0.98	Nodal/Activin Signaling Titrates the Level of Oct4 in ES Cells, Which Is a Direct Target of Phospho-Smad2 Binding in the Promoter Region
	0.98	Nodal/Activin signaling strikingly resemble the ES cell response to a less than 2-fold up- or downregulation of the Oct4 master regulator of stemness in driving differentiation towards similar cell fates.
	0.98	Oct4 expression (Figure 7D) upon inhibition of Nodal/Activin signaling with SB in serum containing media and found that it was also significantly downregulated within 24 hours.
	0.98	Oct4 is a direct target of pSmad2 binding and Nodal/Activin signaling regulates both its promoter activity and endogenous expression.
	0.98	Oct4 is well known for its importance in cell fate decisions and its downregulation during loss of Nodal/Activin signaling is significant not only as an impetus for trophectoderm differentiation but also reconciles the alternative role of Nodal/Activin signaling in maintaining self-renewal and pluripotency.
	0.98	Nodal/Activin pathway starting with different concentrations of ligands external to the ES cell lipid bilayer membrane and terminating with the pSmad2 transcriptional complex regulating Oct4 and different subsets of target genes in the nucleus.
	0.98	Oct4 is itself titrated by the same Nodal/Activin signaling gradients in the ES cells undergoing differentiation.
	0.97	Nodal/Activin signaling in self-renewal and pluripotency versus differentiation cell fate decisions, we examined the regulation of the Oct4 pluripotency and self-renewal master gene.
	0.97	Oct4 may be a key downstream target under Nodal/Activin control during the specification of divergent cell fate decisions and investigated how the pathway may be governing Oct4.
	0.97	Nodal/Activin signaling in chemically defined conditions however, only a pSmad2 peak in the promoter region of Oct4 showed a similarly graded response suggesting that this was the functional Nodal/Activin signaling response element.
	0.97	Oct4 response to graded Nodal/Activin signaling was functionally driven by pSmad2 binding, we determined the exact SBE responsible for Oct4 inducibility (Figure 7C).
	0.96	Oct4 promoter response element with the essential CAGAC SBE was sufficient and independent of all other pSmad2 binding events in the Oct4 locus or other DNA regulatory elements in cis that may be mediated by Nodal/Activin signaling.
	0.79	Oct4 promoter reporter displayed a specific graded response to Nodal/Activin signaling while the control empty reporter did not.
24960041	0.99	Oct4, Klf4, and Activin/Nodal signaling on the expression of Nodal.
	0.98	Oct4 and on Activin/Nodal signaling.
	0.98	Nodal as a tentative direct target of the pluripotency factors Oct4, Sox2, and Nanog in ESCs.
	0.98	Oct4, Nanog, Sox2, and Klf4, in a 2 kb region lying 1 kb upstream of the Nodal transcription start site (TSS) (Figure 1A).
	0.98	Oct4, Oct4 depletion drastically down-regulated the expression of HBE constructs (Figure 3B), suggesting that HBE mediates the influence of Oct4 on Nodal expression.
	0.98	Nodal expression, Oct-4 depletion led to a 5-fold reduction in its response to Activin exposure.
	0.98	Oct4-Smad3 complex bound on HBE could initiate chromatin modifications that would then allow the interaction of ASE with the adjacent promoter, leading to the transcriptional activation of Nodal by the autoregulatory element and the amplification of the Nodal signal.
	0.98	Oct4) and Nodal/Activin signaling activate HBE, which up-regulates Nodal.
	0.92	Nodal declined when the gene encoding Oct4 was knocked down, whereas it was upregulated when Nanog or Sox2 were supressed.
	0.85	Oct4 expression, suggesting they corresponded to differentiating cells in which Nodal expression was driven by other enhancers.
29845790	0.98	Oct4, Nanog, Sox2, Rex1, Dppa3, Tcf3, Utf1, Nodal, Dax1, Sall4 and ss-Catenin) and downregulates early differentiation genes (Gata6, Lefty2 and Cdx2).
	0.97	Oct4, Nanog, Sox2, Rex1, Dppa3, Tcf3, Utf1, Nodal, Dax1, Sall4 and beta-Catenin) and lower expression of early differentiation genes (Gata6, Lefty2 and Cdx2) in R2i condition compared to the serum condition.
	0.82	Oct4 and Dax1), while Nanog, Sox2, Nodal, Dppa3 Tcf3, Rex1, Utf1, and ss-Catenin were upregulated until day 5 and downregulatedafterward.
22710171	0.98	nodal-activated Smad3 in ESC co-occupies the genome with Oct4.
	0.92	Oct4/Smad3 co-ocupied sites are detected in the genome of ESCs exposed to nodal, yet only about 100 genes respond to nodal?
19022741	0.98	Oct4, Fgf4, Gal, Lefty1, Ifitm1, Nodal, Tert, Utf1, Foxd3, Lin28, Grb7, Podxl, and Bxdc2 are expressed during the oocyte to embryo transition.
24319661	0.98	NODAL, BRACHYURY, EOMES, and OCT4.
24560784	0.98	NODAL:that has been shown to serve as an autocrine factor to positively regulate Pou5f1 expression in the spermatogonial cell line C18-4.
25595304	0.98	Nodal signaling: ChIP-seq analysis revealed that SMAD2/3 participates in the control of the many pluripotency factors including Oct4 and Myc.
28455373	0.98	Nodal and Sall4 (Fig. 7B), ZIC2-OTX2 binding regions in EpiSCs were, in the majority of cases, bound by ZIC2 and also by SOX2 plus POU5F1 in ESCs.
28610558	0.98	Nodal maintains the expression of the pluripotency markers Oct4 and Nanog and prevents precocious differentiation of the Epi towards neural lineages.
28669603	0.97	NODAL signaling results in accelerated reduction of OCT4 protein and increase in SOX1 protein at day 3 of differentiation.
	0.94	Nodal, Smad2/3, and Tdgf1 knockdown experiments, the emergence of OCT4-/SOX2+ and SOX2+/SOX1+ cells was accelerated (Figures S3D and S3E).
19619308	0.97	Oct4, Sox2, Klf2, Nanog, Jarid1b, Jarid2, Nodal, Tgif1, and Esrrb, and contains other genes expected to play a role in ES cell function, such as Dppa4 and Dppa5.
24146866	0.97	Pou5f1 (also called Oct4), Nanog, Fgf5, Nodal and Cripto genes, which are expressed dominantly in the epiblast, showed high expression levels in the embryonic samples, while they were almost at the background level in the extraembryonic samples (Figures 1B and S2 in File S1).
27606335	0.97	Oct4, Nodal, Brachyury and Gooscoid over time in the CDM culture system between day 0 and 8, with no expression of the muscle marker Desmin or the epidermal marker pan-Cytokeratin.
20571128	0.95	Nodal messenger RNA (mRNA) in the presence or absence of LIF, SB431542 in the presence of LIF for 96 hours did not lead to any changes in the expression of the genes encoding the pluripotency factors Oct4 and Nanog (Fig. 1D).
	0.93	Oct4 in SB431542-treated cells grown in LIF (Fig. 1D), these genes may not be direct targets of Nodal.
23213411	0.92	Nodal-/- cell lines did not express the pluripotency marker OCT4, which was strongly expressed in ES cells.
	0.84	Nodal mutant embryos do not express the pluripotency marker OCT4.
23681063	0.75	Oct4, Nanog, Eras, Nodal, Sox2 and Esrrb are activated during the intermediate or maturation phase, and genes such as Zfp42, Gdf3, Dppa2, Dppa3 and Utf1 might define the late or stabilization phase.