Publication for Nr0b1 and Pou5f1

Species	Symbol	Function*	Entrez Gene ID*	Other ID	Gene coexpression	CoexViewer
mmu	Nr0b1	nuclear receptor subfamily 0, group B, member 1	11614			[link]
mmu	Pou5f1	POU domain, class 5, transcription factor 1	18999

Pubmed ID	Priority	Text
22369103	0.99	Dax1 binds to Oct4 and abolishes its DNA binding activity, thus decreasing the transcription of Nanog and Rex1, targets of Oct4 activation.
	0.98	Dax1 is an orphan nuclear hormone receptor recently identified as a repressor of Oct4 transcription.
20362541	0.98	Oct4 reduced binding of Tcfcp2l1, Dax1, and Esrrb to several target genes.
	0.98	Dax1 recruitment to the Rest and Nanog promoters, which are both also occupied by many other ESC transcription factors, is dependent on Oct4.
	0.98	Dax1 were shown to cluster across the genome to distinct sets of Oct4 binding sites, suggesting the possibility of cooperativity.
	0.98	Dax1 depends on Oct4 for its binding to the Nanog proximal promoter.
	0.98	Oct4-associated proteins (Sox2, Esrrb, Dax1, Nac1, Klf5, and Zfp143) bind the Nanog proximal promoter, of which at least three do so in an Oct4-dependent manner (Sox2, Esrrb, and Dax1).
	0.97	Oct4, Esrrb, Tcfcp2l1, and Dax1 bind the glycosyl transferase Ogt (O-GlcNAc Transferase; Table 1, Figures 2B-2E), an enzyme that adds N-acetylglucosamine groups (O-GlcNAc) to proteins.
	0.97	Oct4 depletion by 12 hr doxycycline treatment, on the recruitment of Dax1, Tcfcp2l1, and Esrrb to a number of genomic binding sites to which Oct4 also binds.
	0.97	Oct4 reduced recruitment of F-Dax1, F-Tcfcp2l1, and F-Esrrb to several of their targets (Figures 4A-4C).
	0.97	Oct4, Esrrb, Tcfcp2l1, and Dax1 all bind the glycosylating enzyme Ogt, which adds O-GlcNAc groups to proteins.
	0.97	Oct4, Sox2, Dax1, Klf4, Nac1, Esrrb, and Nanog, was found to be almost identical to the Oct4-Sox2 binding site.
	0.96	Oct4 partners Sall4, Tcfcp2l1, Dax1, and Esrrb resulted in an Oct4 interactome of 166 proteins, including transcription factors and chromatin-modifying complexes with documented roles in self-renewal, but also many factors not previously associated with the ESC network.
	0.96	Oct4-interacting transcription factors identified here (Sox2, Nac1, Tcfcp2l1, Esrrb, Dax1) were investigated in those studies and were found to colocalize frequently with Oct4 (Table 2), including at the promoters of important pluripotency genes such as Nanog and Oct4.
	0.89	Oct4 and the parental ZHBTc4 cells have a normal ESC morphology and express normal levels of ESC markers Sox2, Sall4 (Figure S1A available online), Klf4, Dax1, Zfp42, and Eras (Figure S1B).
	0.83	Oct4 with Dax1, Tcfcp2l1, and Esrrb.
21678401	0.98	OCT-4, DAX-1 and the increase in COUP-TFI, COUP-TFII and ETS-1 mRNA levels during the commitment stages of both endodermal and neuronal differentiation.
	0.98	DAX-1, OCT-4 and SF-1 were found to be regulated by PBX in P19 cells treated with RA.
	0.98	OCT-4, DAX-1 and SF-1 expression requires the RA-dependent increase in PBX protein levels.
	0.98	DAX-1 and SF-1 in addition to the well known pluripotency factor OCT-4 may be important regulators of the loss of pluripotency and gain of differentiation specific gene expression in P19 cells.
	0.98	DAX-1 did not block the loss in the mRNA levels of two pluripotency genes, OCT-4 and SF-1, upon treatment with RA in cells induced to differentiate to both endodermal and neuronal cells (Figure 2B).
	0.98	OCT-4, DAX-1 and endogenous SF-1, in a dose-dependent fashion in cells induced to differentiate to both endodermal and neuronal cells with the exception of endogenous SF-1 mRNA levels during neuronal differentiation (Figure 4C).
	0.98	DAX-1, OCT-4 and SF-1) were found to be greatly reduced in wild type cells following RA treatment while their mRNA levels were unchanged in AS2 cells demonstrating that the RA-dependent reduction in the expression of DAX-1, OCT-4 and SF-1 is directly or indirectly regulated by PBX.
	0.98	OCT-4 is well known to be critical for the maintenance of pluripotency while the role of SF-1 and DAX-1 in this process is less well understood.
	0.98	OCT-4, SF-1 and DAX-1 expression in P19 cells induced to differentiate to both endodermal and neuronal cells.
	0.95	DAX-1 expressing cells lost the expression of two markers for pluripotency (SSEA-1 and OCT-4) (Figure 3 A and B) and gained the expression of the endodermal specific marker TROMA-1 (Figure 3A) and the neuronal specific marker TUJ1 (Figure 3B).
	0.86	OCT-4, TROMA-1 and TUJ1 expression seven days after RA treatment demonstrated that overexpression of DAX-1 did not affect the ability of these cells to differentiate to either endodermal cells or neuronal cells.
28548056	0.98	Oct4 through interacting with another orphan nuclear receptor, Dax1.
	0.98	Dax1 cooperated with Nr5a2 or Oct4 proteins to control Oct4 transcription.
	0.98	Dax1 is believed to regulate Oct4 expression through modulating the activity of Nr5a2 or Oct4 proteins, which bind directly to the corresponding DNA motifs in the Oct4 promoter and enhancers.
	0.97	Oct4 expression through interacting with other factors, such as Dax1, RARgamma and Nanog; whereas, the relationship among these Nr5a2 binding partners still remains unclear.
	0.97	Dax1 was found to associate with Oct4 proteins through the POU-domain.
	0.97	Oct4 regulated Dax1 transcription through a putative binding site at -158 bp and +2054 bp, which are located in Dax1 promoter and an intronic region respectively.
	0.97	Oct4, Sox2 and Nanog, proteomics studies suggested that Sall4 could also associate with other transcription factors, such as Esrrb, Dax1, MTA2 (NuRD complex component) and Nac1 in mouse ESCs, and linked to TGF-beta and WNT signaling through interacting with Usp9X and Cxxc5.
	0.92	Oct4, Nanog, Dax1, NacI, and Klf4 in the Rex1 promoter.
	0.86	Oct4, Dax1 was also identified to interact with Nanog in a proteomics analysis, yet its functional significance has not been addressed.
29845790	0.98	Oct4, Nanog, Sox2, Rex1, Dppa3, Tcf3, Utf1, Nodal, Dax1, Sall4 and ss-Catenin) and downregulates early differentiation genes (Gata6, Lefty2 and Cdx2).
	0.98	Dax1 in ESC, led to an increased expression of Oct4.
	0.98	Oct4 can bind to the promoter region of Dax1 and regulate its expression level.
	0.97	Oct4, Nanog, Sox2, Rex1, Dppa3, Tcf3, Utf1, Nodal, Dax1, Sall4 and beta-Catenin) and lower expression of early differentiation genes (Gata6, Lefty2 and Cdx2) in R2i condition compared to the serum condition.
	0.83	Oct4 and Dax1), while Nanog, Sox2, Nodal, Dppa3 Tcf3, Rex1, Utf1, and ss-Catenin were upregulated until day 5 and downregulatedafterward.
21062744	0.98	Oct4, and Sox2 acting as the master regulators, whereas other factors such as Nr0b1, Sall4, c-Myc, Klf4, Zic3, Esrrb, Tcf3, Suz12, Zfp206, and Zfp281 also have important roles in the maintenance of stem cell identity.
	0.97	Oct4, Sox2 Sall4, Nr0b1, and Sall1 itself and many differentiation-related genes such as Hox genes, T brachyury, Isl1, Hand1, and Otx2 (Fig. 4D) are included in the aforementioned categories, suggesting that Sall1 is part of an elaborate network of cross-regulated factors.
	0.92	Oct4, Nr0b1, and Sall4) and Sall1 itself, whereas inactive genes were mostly associated with differentiation and development.
	0.66	Oct4, and Nr0b1.
25227241	0.98	Oct4-centered PPI network was also defined by multiple independent research groups; van den Berg et al. showed that Oct4 is physically associated with 166 proteins, including TFs (Sall4, Tcfcp2l1, Dax1, and Esrrb) and chromatin modifiers (SWI/SNF and NuRD complexes) that are implicated in the self-renewal of ES cells.
	0.98	Oct4, Sox2, Klf4, and cMyc) and some Nanog-associated TFs (Nanog, Dax1, Rex1, Zfp281, and Nacc1) revealed that target genes bound by few factors tested are in general inactive, whereas common targets of many TFs are mostly active in ES cells.
	0.98	Oct4, Sox2 and Nanog, as well as other TFs, such as Smad1, Stat3, Nacc1, Dax1, and Zfp281, form the Core class TFs, and mainly co-occupy distal regulatory elements of mouse ES cells.
25772165	0.98	Nr0b1 is regulated by the LIF signal via Jak-Stat3 pathway as well as by Oct3/4, Nanog, Nr5a2 and Esrrb.
	0.98	Nr0b1 binds to Oct3/4 to inhibit its transcriptional activity and the over-expression of Nr0b1 induces differentiation toward trophectoderm as in the case of Oct3/4 knockout.
	0.98	Nr0b1 interacts with Nr5a2 to suppress its function, it was also demonstrated that Nr0b1 cooperates with Nr5a2 and Steroid receptor RNA activator 1 (Sra1) to activate the Oct3/4 promoter.
20687156	0.98	Oct4 target genes, including Nanog, FGF-4, and Lefty-1, and other genes important for the self-renewal of ESC, such as Dax1 and Sall4, exhibited decreased expression after differentiation.
	0.98	Oct4 gene expression, and Dax1, a negative regulator of the transcriptional activity of Oct4, both decrease at the RNA level as early as 9 hours after Dox induction of Sox21 (Supplemental Fig. 5).
21156086	0.98	Oct4 interacts with other pluripotency factors - such as Nanog, Sox2, Sall4, Klf5, Zfp143, Zfp206, Esrrb, Dax1 and Tcfcp2l1 - to form auto-regulatory and cross-regulatory loops and to maintain a pluripotent state.
	0.98	Dax1, depend on Oct4 for the efficient targeting of several of their shared sites, emphasizing the importance of coordination among pluripotency factors and the critical role of Oct4 in the maintenance of a pluripotent state.
25890371	0.98	Nr0b1, Myb, Zic3, and Pou5f1, were found to be involved in the conversion of the mouse fibroblasts into the iNSCs (Table 2).
	0.71	Pou5f1, Sall4, Nanog, Jarid2, Suz12, Ezh2, Sox2, Rad21, Mtf2, Tfcp2l1, Nr0b1, Smarca4, Tet1, Olig2, and Tcf3 were found to be present in at least two out of three networks analyzed in the current study.
26057209	0.98	Oct4, Sox2), revealed that TFs including Tcfcp2l1, Stat3, Dax1, and Klf4, are important members of a larger network of regulators securing pluripotency or maintenance of the undifferentiated state in murine embryonic stem (mES) cells.
	0.93	Dax1 and Sall4 lead to a loss of pluripotency, as assessed by loss of Oct4 and derepression of certain lineage markers, loss of Nac1 or Zfp281 did not alter the expression of the stem-cell markers Nanog and Oct4.
27281610	0.98	NR0B1 is regulated by STAT3, OCT3/4, NANOG, NR5A2 and estrogen receptor beta (ERbeta), all of which are essential transcription factors for ES cell self-renewal.
	0.93	NR0B1 is under the control of a cross-talking network that involves transcriptional factors such as NANOG, OCT3/4, androgen receptor (AR), NR5A1 (also named steroidogenic factor 1), NR5A2 (also named liver receptor homolog-1), SOX2 and STAT3 during embryogenesis in addition to the production of steroid hormones, we next analyzed changes in these factors in 293T cells that were treated with TSA.
31953406	0.98	Pou5f1/Oct4, Sox2, and Nanog remained unchanged in response to siSrrt, some examples of this category (e.g. Nr0b1, Pecam1, and Zic2) were detectably downregulated (Supplementary Fig. 2b).
	0.96	Pou5f1, Sox2, Nanog, and Zfp42/Rex1, it leads to significant downregulation of Nr0b1, Pecam1, and Zic2 and upregulation of early differentiation markers Etv4, Otx2, and Runx1 (refs. ).
19785031	0.98	Oct4, Sox2, Nanog, Esrrb, Tbx3, Tcl1, Nac1, Dax1, Zfp281, and Zic3, which participate in auto- and cross-regulatory interactions to increase their own expression and that of other self-renewal genes.
21663790	0.98	Oct4, Sox2, and Nanog constitute bona fide core factors, additional ecat, such as Dax-1, Rex1, Sall4, and Tcl1, function within a larger regulatory network supporting pluripotency.
24672749	0.98	Oct4, Sox2, Nanog, and other reprogramming markers, such as Eras and Dax1, progressively increased in OySyNyK-transduced MEFs from day 2 to 6 as shown by qRT-PCR (Figures 1H and S2B).
24736627	0.98	Oct3/4, Klf4, Sox2, Rex1, Esrrb, Dax1, and Lrh1 (Fig. 4B).
24792115	0.98	Oct4, Nanog, Sox2, Klf4, Esrrb, Tcl1, Tbx3, Nr0b1, Nr5a2, Foxd3, Zfp42, and Tet1 (Figure 2B, S3C).
25408886	0.98	Oct4 interaction protein network have revealed that the Oct4 interactome includes many transcription factors and chromatin-modifying complexes with documented roles in self-renewal and pluripotency, and that acute depletion of Oct4 reduces the binding of Tcfcp2l1, Dax1, and Esrrb to target genes .
26206133	0.98	Nr0b1 is part of the ES cell self-renewal network where it interacts with Oct4 and gets recruited to Oct4/Sox2-binding sites.
26431182	0.98	Oct4/Pou5f1, Sox2, and Zfp42), genes involved in DNA methylation (Dnmt3a, Tet1, and Tet2), and other genes such as nuclear receptor Nr0b1 and histone lysine acetyltransferase Kat6b.
26943822	0.98	Oct3/4 exists in a large complex containing Sall4, Tcfcp2l1, Dax1, Esrrb, and other epigenetic modifiers in mouse ES cells.
26971889	0.98	Dax1, Lrh1 activates the transcription of Oct4, which encodes the key factor for maintaining the pluripotency of mouse ESCs.
27921030	0.98	OCT4, SOX2, NANOG (and others, e.g., KLF4, c-MYC, Dax1, Rex1, Zpf281, and Nac1).
29445086	0.98	POU5F1, SOX2, NANOG, ESRRB, SALL4, PRDM14, TCF3, MYC, NR0B1, YY1, ZFX, SMC1A, KDM5B, E2F1, E2F4, ZFP42, MAX, NIPBL, ASH2L, MED1, MED12, and ZIC3 (Fig. 4b).
23401375	0.97	Oct4, Dax1, Zfp281, and Nac1 are each part of large protein complexes (some >1 MDa) that contain many other transcription factors, chromatin-modifying machinery and co-activators.
	0.97	Oct4, Dax1, Nac1, Zfp281 and Rex1 not only associate with one another in ESC, they also associate with other transcription factors in common.
	0.95	Oct4, Sox2, Nanog, Klf4, c-Myc, Dax1, Rex1, Nac1 and Zpf281, as well as the genome-wide distribution of two histone modifications, histone 3 lysine 4 trimethylation (H3K4me3) and histone 3 lysine 27 trimethylation (H3K27me3), which are associated with actively transcribed genes and repressed genes, respectively.
	0.89	Oct4, Sox2, Nanog, Sall4, Tcfcp2l1, Esrrb, Nac1, Dax1, Zfp281 and Rex1 were integrated to produce a more complete picture of the protein-protein interaction landscape in ESC.
	0.58	Oct4, Dax1, Nac1, Zfp281 and Rex1).
23468881	0.97	Pou5f1, Sox2, Nanog, Nr0b1, and Rif1, (Figure 4A, Figure S5).
	0.83	Pou5f1, Sox2, Nanog, Nr0B1, and Phc1.
	0.63	Nr0b1, Phc1, Pou5f1, and Sox2.
23876535	0.97	Pou5f1; also known as octamer binding transcription factor 4 or Oct4), zinc finger protein 296 (Zfp296), dosage-sensitive sex reversal nuclear receptor subfamily 0 group B member 1 (Nr0b1; also commonly referred to as Dax1), undifferentiated embryonic cell transcription factor 1 (Utf1), Nanog and SRY-box containing gene 2 (Sox2) were detectable in ESCs and PGCs, whereas SSCs expressed only Pou5f1, Zfp296, Nr0b1 and Utf1 (Fig. 1B).
	0.97	Pou5f1 was detectable in freshly isolated OSCs; however, ex vivo culture of OSCs resulted in activation of all pluripotency-associated genes with the exception of Nr0b1 (Fig. 1B).
23892456	0.97	Oct4/Esrrb/Sall4/Nr0b1/Tcfcp2l1 interactome, do interact with Nanog (Figure 2).
	0.95	Oct4, Zfp281, Nac1, Rex1 and Nr0b1.
18274628	0.97	DAX1 similar to that observed for Oct-4.
23653415	0.97	Dax1, Nac1, and Oct4 preferentially interacted with wild-type Nanog versus the monomeric Nanog10WA.
23724002	0.97	Pou5f1, Nr0b1, Zfp281, Sall4, Gadd45a, Bmpb8a, Ascl2, Dppa5a, Zic1, Cebpb, Cxcl12 and Zfp42).
25173149	0.97	Dax1, and Zfp281, Oct4 and Wdr5, Tet1 and Sin3a, Tet1/2 and Ogt, and Hdac1/2 and NuRD.
27130680	0.97	Pou5f1, Eed, Phc1, Myc, Fgf4, Lefty1, Nr0b1, and Dnmt3b) compared to standard ESCs (Fig. 2A).
27281218	0.97	Pou5f1, Nr0b1 (Dax1), Lin28a and Myc were downregulated, whereas lineage commitment genes such as Elf3, Cdx1, and Pitx2 were upregulated.
18394158	0.96	Oct4, and other genes typical of the undifferentiated state, such as Rex1/Zfp42, FoxD3, Gdf3, Nr0b1, Eras, Rif1, Tbx3 and Esrrb .
26611864	0.96	Oct4, Nanog, and Sox2) and lack Esg1 and Dax1 expression when compared to ESCs.
27991575	0.95	Pou5f1/Oct4, Sox2, and Nr0b1.
19662495	0.94	Oct4, Nac1, Rif1, Dax1, and Zfp281, by co-immunoprecipitation.
20734354	0.86	Dax-1/Nr0b1, and liver receptor homolog-1 (Lrh-1)/Nr5a2 have been reported to comprise secondary pluripotency regulation, however, these studies are limited in that they focus on Oct4 regulation alone or do not report a clear mechanism.
26740582	0.85	Nr0b1 (also known as Dax1), Nr5a2, Oct4, Prdm5, Sall1, Sox2, Tfcp2l1, Utf1, Zfp462, Zic3 and Zscan10 (also known as Zfp206) (see Supplementary Table S4 for more detailed information about these genes).
22355722	0.83	Oct4, Sox2, Nanog, Rex1, and Dax1), ALP-staining, embryoid body (EB) formation, and the ability to differentiate into cells of three germlayers (Fig. 2d-2k).
23789112	0.78	Dax1 (NrOb1), Dazl, Essrb, Fbxo15, Fgf4, Gbx2, Klf4, Lefty2, Nanog, Oct4 (Pou5f1), Otx2, Pecam1, Pitx2, Piwil2, Rex1 (Zfp42), Tbx3, and Tcfcp2l1], the mRNA levels of which have been used to evaluate stemness and demarcate the naive and primed pluripotent states in rodent cells.
24859004	0.71	Pou5f1, Gdf3, Tfcp2l1 and Nr0b1 (Dax1) (Fig.1c,d).
29721022	0.69	Oct4, Sox2, Klf4, c-Myc, Nanog, Utf1, Fgf4, Esg1, Gdf3, Zfp296, Cripto, Dax1, Neo, and Nat1) with F-iPS clones and ESCs.
26832399	0.66	Oct4, Sox2, Nanog, Klf4, cMyc, Nac1, Dax1, Rex1 and Zfp281) and others (Tcf3, Klf5, p53 and Tbx3) which are thought to have various roles in regulating pluripotency.
27664156	0.63	Oct4, Sox2, Nanog, Klf4, Zfp296, Dax1, Esg1, and Eras revealed no significant differences (P > 0.05) from those of wild type mESCs (Fig. 2C).